ReadSim

 ReadSim- A Simulator for Sanger and 454 Sequencing

New sequencing technology based on pyro-sequencing (as recently introduced

by 454 Life Sciences) promises to be ideally suited for metagenomic projects that seek to determine the diversity of organisms in a given environment. To develop and calibrate software to process the arising data, a sequencing simulator is required that samples reads from a given known target sequence under an error model that reflects the performance of the sequencing technology. Additionally, to simplify the comparison of pyro-sequencing and Sanger sequencing, it is desirable that both approaches can be simulated by a single program.

Here we provide a sequencing simulator ReadSim that simulates the sequencing of reads both for Sanger and pyro-sequencing, optionally involving mate-pairs.

ReadSim is no longer maintained.

All concepts of ReadSim are integrated into our new software MetaSim. (Oct 2008)

Use of the program is free for academic purposes at an academic institute. For all other uses, please contact the authors.

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Manual

Authors

The program was written by Ramona Schmid and Daniel Huson